#3595 DIALYSIS MODALITY AFFECTS SENESCENCE-RELATED PHENOTYPE OF T AND B LYMPHOCYTES

Abstract Background and Aims Hemodiafiltration (HDF) is characterized by enhanced clearance of middle molecular weight toxins therefore, it considered superior to hemodialysis (HD), especially in higher hemofiltration volumes. Although proinflammatory cytokines are lower HDF, its impact on immune phenotype has not been studied, so far. In this study we compared the lymphocytes’ senescent between HD HDF patients. Method Senescent exhausted related lymphocyte markers, including CD45RA, CCR7, CD28, CD57 PD1 T cells CD27 IgD B cells, were assessed flow cytometry 35 ESRD patients 25 similar age, sex, ethnicity. Moreover, evaluated association these markers with volume. Results Patients had significantly reduced CD4+CD28- lymphocytes, 31(18-74) vs 50(36-131)cells/μL, respectively, p = 0.019, which was restricted only CD28-CD57- compartment, [17(9-25) 31(19-50)cells/μL, 0,002], while terminally differentiated CD28-CD57+ did differ two groups [14(4-49) 23(7-52)cells/μL, 0,22]. Expression molecule CD4+ depended dialysis vintage (r 0.27, 0.03). percentage CD4+PD1+ [8.5(6.8-11.7) 13.7(8.7-19.5) % for 0.006]. CD8+PD1+ also patients, without reaching statistical significance [17.4(8.5-41) 34(12,7-52.9) 0.06]. Hemofiltration volume negatively correlated effector memory CD8+ re-expressing CD45RA count (CD8+ EMRA), -0.46, 0.027) positively total 0.46, 0.025). However, all cell subsets equally affected, naïve IgD+CD27- switched IgD-CD27+ showing positive correlation 0.53, 0.008 r 0.5, 0.015, respectively). Conclusion high filtration volumes may have beneficial effects adaptive immunity, predisposing less phenotypes.